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foxp3 treg cell induction  (R&D Systems)


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    R&D Systems foxp3 treg cell induction
    FIGURE 2. CD69 surface expression is induced by oral Ag challenge in TCR transgenic mice. B6 (A) and OT-II 3 RAG2/2 (B) mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. The expression of the activation Ags CD69 and CD25 by CD4 T cells from spleen (S), MLN, siLP, and cLP was analyzed by multicolor FCM. Data from an individual representative mouse (out of four mice analyzed) are shown. Numbers indicate the percentage of CD4 T cells that express the activation Ags CD69 or CD25. C, OT-II 3 RAG2/2 mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. In- tracellular expression of T-bet, GATA-3, RORgt, and <t>Foxp3</t> and surface expression of IFN-gR, CD122, CD103, TGF-bRII, IL-21R, IL-10R, ICOS, and LAP/TGF-b1 gated on CD69+ and CD692 CD4 T cells from siLP were analyzed by multicolor FCM. Filled curves represent the respective negative controls. Four mice were analyzed, and the data from a representative individual mouse are presented. Numbers represent the percentage of cells that are positive for the indicated Ag.
    Foxp3 Treg Cell Induction, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/foxp3+treg+cell+induction/FoxP3/pm22250092-109-5-15
    Average 94 stars, based on 98 article reviews
    foxp3 treg cell induction - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential."

    Article Title: CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1100765

    FIGURE 2. CD69 surface expression is induced by oral Ag challenge in TCR transgenic mice. B6 (A) and OT-II 3 RAG2/2 (B) mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. The expression of the activation Ags CD69 and CD25 by CD4 T cells from spleen (S), MLN, siLP, and cLP was analyzed by multicolor FCM. Data from an individual representative mouse (out of four mice analyzed) are shown. Numbers indicate the percentage of CD4 T cells that express the activation Ags CD69 or CD25. C, OT-II 3 RAG2/2 mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. In- tracellular expression of T-bet, GATA-3, RORgt, and Foxp3 and surface expression of IFN-gR, CD122, CD103, TGF-bRII, IL-21R, IL-10R, ICOS, and LAP/TGF-b1 gated on CD69+ and CD692 CD4 T cells from siLP were analyzed by multicolor FCM. Filled curves represent the respective negative controls. Four mice were analyzed, and the data from a representative individual mouse are presented. Numbers represent the percentage of cells that are positive for the indicated Ag.
    Figure Legend Snippet: FIGURE 2. CD69 surface expression is induced by oral Ag challenge in TCR transgenic mice. B6 (A) and OT-II 3 RAG2/2 (B) mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. The expression of the activation Ags CD69 and CD25 by CD4 T cells from spleen (S), MLN, siLP, and cLP was analyzed by multicolor FCM. Data from an individual representative mouse (out of four mice analyzed) are shown. Numbers indicate the percentage of CD4 T cells that express the activation Ags CD69 or CD25. C, OT-II 3 RAG2/2 mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. In- tracellular expression of T-bet, GATA-3, RORgt, and Foxp3 and surface expression of IFN-gR, CD122, CD103, TGF-bRII, IL-21R, IL-10R, ICOS, and LAP/TGF-b1 gated on CD69+ and CD692 CD4 T cells from siLP were analyzed by multicolor FCM. Filled curves represent the respective negative controls. Four mice were analyzed, and the data from a representative individual mouse are presented. Numbers represent the percentage of cells that are positive for the indicated Ag.

    Techniques Used: Expressing, Transgenic Assay, Activation Assay

    FIGURE 4. Reduced Foxp3+ Treg cell number in the absence of CD69. A, Cells were isolated from spleen (S), MLN, siLP, and cLP of nontreated B6 and CD692/2 mice and analyzed for intracellular expression of Foxp3 in CD4 T cell population by multicolor FCM. Numbers indicate the percentage of CD4+
    Figure Legend Snippet: FIGURE 4. Reduced Foxp3+ Treg cell number in the absence of CD69. A, Cells were isolated from spleen (S), MLN, siLP, and cLP of nontreated B6 and CD692/2 mice and analyzed for intracellular expression of Foxp3 in CD4 T cell population by multicolor FCM. Numbers indicate the percentage of CD4+

    Techniques Used: Isolation, Expressing

    Related Articles

    Cell Culture:

    Article Title: CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential.
    Article Snippet: .. To simulate conditions that favor Foxp3+ Treg cell induction, 2000 U/ml IL-2 (catalog no. 402-ML; R&D Systems) and 5 ng/ml TGF-b1 (catalog no. 240-B; R&D Systems) were added to the cell culture. .. After 5 d culture, cells were washed with PBS supplemented with 0.3% BSA and 0.1% sodium azide, stained for surface CD4 with allophycocyanin-conjugated mAb binding CD4 GK1.5 (catalog no. 17-0041- 83; eBioscience), intracellular for Foxp3 with FITC-conjugated anti-Foxp3 Ab FJK-16s (catalog no. 11-5773-82; eBioscience), and analyzed with FCM.



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    foxp3 treg cell induction  (R&D Systems)
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    R&D Systems foxp3 treg cell induction
    FIGURE 2. CD69 surface expression is induced by oral Ag challenge in TCR transgenic mice. B6 (A) and OT-II 3 RAG2/2 (B) mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. The expression of the activation Ags CD69 and CD25 by CD4 T cells from spleen (S), MLN, siLP, and cLP was analyzed by multicolor FCM. Data from an individual representative mouse (out of four mice analyzed) are shown. Numbers indicate the percentage of CD4 T cells that express the activation Ags CD69 or CD25. C, OT-II 3 RAG2/2 mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. In- tracellular expression of T-bet, GATA-3, RORgt, and <t>Foxp3</t> and surface expression of IFN-gR, CD122, CD103, TGF-bRII, IL-21R, IL-10R, ICOS, and LAP/TGF-b1 gated on CD69+ and CD692 CD4 T cells from siLP were analyzed by multicolor FCM. Filled curves represent the respective negative controls. Four mice were analyzed, and the data from a representative individual mouse are presented. Numbers represent the percentage of cells that are positive for the indicated Ag.
    Foxp3 Treg Cell Induction, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/foxp3+treg+cell+induction/FoxP3/pm22250092-109-5-15
    Average 94 stars, based on 1 article reviews
    foxp3 treg cell induction - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

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    FIGURE 2. CD69 surface expression is induced by oral Ag challenge in TCR transgenic mice. B6 (A) and OT-II 3 RAG2/2 (B) mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. The expression of the activation Ags CD69 and CD25 by CD4 T cells from spleen (S), MLN, siLP, and cLP was analyzed by multicolor FCM. Data from an individual representative mouse (out of four mice analyzed) are shown. Numbers indicate the percentage of CD4 T cells that express the activation Ags CD69 or CD25. C, OT-II 3 RAG2/2 mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. In- tracellular expression of T-bet, GATA-3, RORgt, and Foxp3 and surface expression of IFN-gR, CD122, CD103, TGF-bRII, IL-21R, IL-10R, ICOS, and LAP/TGF-b1 gated on CD69+ and CD692 CD4 T cells from siLP were analyzed by multicolor FCM. Filled curves represent the respective negative controls. Four mice were analyzed, and the data from a representative individual mouse are presented. Numbers represent the percentage of cells that are positive for the indicated Ag.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential.

    doi: 10.4049/jimmunol.1100765

    Figure Lengend Snippet: FIGURE 2. CD69 surface expression is induced by oral Ag challenge in TCR transgenic mice. B6 (A) and OT-II 3 RAG2/2 (B) mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. The expression of the activation Ags CD69 and CD25 by CD4 T cells from spleen (S), MLN, siLP, and cLP was analyzed by multicolor FCM. Data from an individual representative mouse (out of four mice analyzed) are shown. Numbers indicate the percentage of CD4 T cells that express the activation Ags CD69 or CD25. C, OT-II 3 RAG2/2 mice were fed for 2 d with 1 mg OVA protein dissolved in PBS. In- tracellular expression of T-bet, GATA-3, RORgt, and Foxp3 and surface expression of IFN-gR, CD122, CD103, TGF-bRII, IL-21R, IL-10R, ICOS, and LAP/TGF-b1 gated on CD69+ and CD692 CD4 T cells from siLP were analyzed by multicolor FCM. Filled curves represent the respective negative controls. Four mice were analyzed, and the data from a representative individual mouse are presented. Numbers represent the percentage of cells that are positive for the indicated Ag.

    Article Snippet: To simulate conditions that favor Foxp3+ Treg cell induction, 2000 U/ml IL-2 (catalog no. 402-ML; R&D Systems) and 5 ng/ml TGF-b1 (catalog no. 240-B; R&D Systems) were added to the cell culture.

    Techniques: Expressing, Transgenic Assay, Activation Assay

    FIGURE 4. Reduced Foxp3+ Treg cell number in the absence of CD69. A, Cells were isolated from spleen (S), MLN, siLP, and cLP of nontreated B6 and CD692/2 mice and analyzed for intracellular expression of Foxp3 in CD4 T cell population by multicolor FCM. Numbers indicate the percentage of CD4+

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential.

    doi: 10.4049/jimmunol.1100765

    Figure Lengend Snippet: FIGURE 4. Reduced Foxp3+ Treg cell number in the absence of CD69. A, Cells were isolated from spleen (S), MLN, siLP, and cLP of nontreated B6 and CD692/2 mice and analyzed for intracellular expression of Foxp3 in CD4 T cell population by multicolor FCM. Numbers indicate the percentage of CD4+

    Article Snippet: To simulate conditions that favor Foxp3+ Treg cell induction, 2000 U/ml IL-2 (catalog no. 402-ML; R&D Systems) and 5 ng/ml TGF-b1 (catalog no. 240-B; R&D Systems) were added to the cell culture.

    Techniques: Isolation, Expressing